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NEW GENERATION DIAGNOSIS SYSTEMS FOR GMO
DETECTION |
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Nanoparticle Based
Biosensors
QCM Based Detection Systems |
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PLANT GENOMICS VIA MICROARRAY ANALYSIS
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Transcriptome Analysis of Crop
Plants Under Abiotic Stress Conditions
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GENE EXPRESSION LEVEL ANALYSIS UNDER
ABIOTIC STRESS CONDITIONS |
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Stress
Types: Salinity, Drought, Cold
Target Plants: Wheat,
Barley
Target
Genes: Superoxide dismutase gene, Trehalose-6-phosphate
synthase gene, Trehalase gene
Techniques:
Northern Blot, Quantitative Real Time PCR (Q-PCR), Reverse
Transcriptase PCR (RT-PCR)
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● GENETIC MANIPULATION OF CROP PLANTS
Target Plant Species
Tobacco (model)
Potato
Wheat
Lentil
Chickpea
Sugarbeet
Target Explants
Mature-immature embryo & infloresences
Cotyledonary nodes
Leaf, cotyledon, stem
Transformation Techniques
Agrobacterium mediated
Microprojectile bombardment (Wheat, Lentil)
Interested Agronomic Characters
Abiotic stress resistance
(salt & drought - P5CS, proline, aldose reductase, STO, CBF1,
transcription factors)
Microbial resistance (pflp)
Delayed ripening
(ACC oxidase, antisense)
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● STRESS PHYSIOLOGY & BIOCHEMISTRY
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Physiological and biochemical aspects of abiotic stress resistance in
crop plants
Stress Types:
boron toxicity, heat, cold, salt, drought, cadmium toxicity
Target Plant: wheat, lentil, barley,
sunflower, chickpea
Techniques:
1D-2D Electrophoresis, Photosystem-II Activity Measurements, Membrane
Leakage Tests (CMS), TTC Viability Tests, Kinetic Analysis of Antioxidant
Defense System Enzymes (SOD, Ascorbate peroxidase, Catalase, Glutathion reductase), H2O2, MDA,
Proline measurements.
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MICROTUBER PRODUCTION IN POTATO |
Major aim is to produce
microtubers tubers from cultured potato shoots using bioreactors
and conventional tissue culture methods.
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